详细信息
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10
FN
VR
PT
J
AU
She, RC Taggart, EW Petti, CA
AF
She, Rosemary C. Taggart, Edward W. Petti, Cathy A.
TI
Comparison of 10 Indirect Fluorescent Antibodies to Detect and Type Influenza A Specimens
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ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE
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English
DT
Proceedings Paper
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23rd Annual Clinical Virology Symposium
CY
APR 29-MAY 02, 2007
CL
Clearwater, FL
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HO
DE
ID
MONOCLONAL-ANTIBODIES; RAPID INFLUENZA; MULTIPLEX PCR; UNITED-STATES; B VIRUSES; MANAGEMENT; SUBTYPES; IDENTIFICATION; SPECIFICITY; DIAGNOSIS
AB
Context.-Management of influenza infections relies on rapid, accurate, and sensitive diagnostic techniques. Influenza A (IA) strain typing has become more important since the emergence of highly pathogenic avian and novel influenza strains and the high frequency of oseltamivir resistance in circulating H1N1 isolates. Objective.-To analyze the performance of indirect fluorescent antibody testing for subtyping a broad range of IA strains. Design.-Ten indirect fluorescent antibody reagents were used to detect and type 100 archived IA respiratory specimens from 1986 through 1995 and 2006 through 2007 and a reassortant, nonpathogenic H5N1 sample. Both direct specimen and cultured isolates were tested. Reverse transcription-polymerase chain reaction was used to confirm indirect fluorescent antibody results. Three H1N1-, 2 H3N2-, and 1 H1-H2-H3-H5-specific antibodies (Chemicon Diagnostics), an IA pool reagent (Trinity Biotech), and H1, H3, and H1-H3-specific antibodies (Centers for Disease Control and Prevention) were used. Results.-Reverse transcription-polymerase chain reaction confirmed all 100 isolates as IA and identified 71 as H1, 22 as H3, and 7 as non-H1-H3. Sensitivity of direct specimen testing ranged was 18.3% to 57.7% for the H1 reagents, 36.4% to 50.0% for the H3 reagents, and 40.0% to 53.8% for the pool reagents. Subtyping was more sensitive on cultured isolates than direct specimens. Specificity for all antibodies was 89.7% to 100%. The H5N1 sample was positive by direct testing and culture (reverse transcription-polymerase chain reaction, Centers for Disease Control and Prevention H5N1 pool, Chemicon H1-H2-H3-H5). No cross-reactivity was observed when the 10 antibodies were tested against other common respiratory viruses. Conclusions.-When positive, IA subtyping antibodies can serve as a useful diagnostic tool when multiple influenza virus subtypes are cocirculating with different susceptibility patterns. (Arch Pathol Lab Med. 2010; 134: 1177-1180)
C1
1 [She, Rosemary C.; Petti, Cathy A.] Univ Utah, Sch Med, Dept Pathol, Salt Lake City, UT 84108 USA. [Petti, Cathy A.] Univ Utah, Sch Med, Dept Med, Salt Lake City, UT 84108 USA. [She, Rosemary C.; Taggart, Edward W.; Petti, Cathy A.] Associated Reg & Univ Pathologists Labs, Salt Lake City, UT USA.
RP
She, RC, Univ Utah, Sch Med, Dept Pathol, 500 Chipeta Way, Salt Lake City, UT 84108 USA.
EM
rosemary.she-bender@hsc.utah.edu
CR
ALVAREZ AC, 2008, VIROL J, V5, ARTN 77 BHAVNANI D, 2007, INT J INFECT DIS, V11, P355, DOI 10.1016/j.ijid.2006.09.009 BRAMMER L, 2008, MMWR-MORBID MORTAL W, V57, P1046 BRAMMER L, 2009, MMWR-MORBID MORTAL W, V58, P115 FALSEY AR, 2007, ARCH INTERN MED, V167, P354 JENNINGS LC, 2009, INFLUENZA OTHER RESP, V3, P91, DOI 10.1111/j.1750-2659.2009.00079.x MAHONY J, 2007, J CLIN MICROBIOL, V45, P2965, DOI 10.1128/JCM.02436-06 NOYOLA DE, 2000, PEDIATR INFECT DIS J, V19, P303 SCHMIDT NJ, 1982, J CLIN MICROBIOL, V16, P763 STEVENS J, 2008, J MOL BIOL, V381, P1382, DOI 10.1016/j.jmb.2008.04.016 STOCKTON J, 1998, J CLIN MICROBIOL, V36, P2990 TKACOVA M, 1997, J CLIN MICROBIOL, V35, P1196 UEDA M, 1998, J CLIN MICROBIOL, V36, P340 VARECKOVA E, 2002, J CLIN MICROBIOL, V40, P2220 VARECKOVA E, 2008, VIRUS RES, V132, P181, DOI 10.1016/j.virusres.2007.10.004 WALLS HH, 1986, J CLIN MICROBIOL, V23, P240 WRIGHT KE, 1995, J CLIN MICROBIOL, V33, P1180 ZIEGLER T, 1995, J CLIN MICROBIOL, V33, P318
NR
18
TC
0
PU
COLLEGE AMER PATHOLOGISTS
PI
NORTHFIELD
PA
C/O KIMBERLY GACKI, 325 WAUKEGAN RD, NORTHFIELD, IL 60093-2750 USA
SN
0003-9985
J9
9 ARCH PATHOL LAB MED
JI
Arch. Pathol. Lab. Med.
PD
AUG
PY
2010
VL
134
IS
8
BP
1177
EP
1180
DI
PG
4
SC
Medical Laboratory Technology; Medicine, Research & Experimental; Pathology
GA
635DU
UT
ISI:000280636600016
ED
SU
BN
SE
CA
SI
AR
GP
PN
BA